SALIVARY AND SERUM ENZYMES AS DIAGNOSTIC BIOMARKERS IN PATIENTS WITH PERIODONTAL DISEASE

Introduction: Periodontal disease is one of the most widespread oral disease.

Aim: The general aim of the present study was to detect salivary and serum enzyme activities in response to periodontal infection, involving the release of enzymes from stromal, epithelial, inflammatory and bacterial cells.

Methodology: We included 20 patients with chronic periodontitis and 20 controls. Unstimulated whole saliva and serum was used to detect the following enzymes: aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and gamaglutamil transferase (GGT). Periodontal status was established using classic clinical parameters, plaque index, bleeding index and probing depth.

Results:  In patient’s saliva with periodontal disease we detected significantly decreased levels of  LDH  and ALP. Salivary level for GGT was decreased and for AST were increased but no statistical significance was found when compared to controls. Significantly increased levels for serum ALP and GGT were observed in patients with periodontal disease. At the same time no statistical difference was found between controls and periodontitis patients for AST and LDH.

Discussions: Our results reflect changes of intracellular enzymes both in saliva and serum, at patients with periodontal disease group versus healthy subjects.

Conclusions: The activity of this enzymes in saliva and serum of patients with periodontal disease can be useful in diagnosis, monitorization and treatement of this inflammatory disease.

Keywords: saliva, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, gamaglutamil transferase, periodontitis

Authors: Daniela Miricescu, Alexandra Totan, Bogdan Calenic, Brânduşa Mocanu, Maria Greabu
DOI: https://doi.org/10.25241/stomaeduj.2014.1(1).art.4

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