Cytotoxicity of indirect restorative materials on fibroblast cells: in-vitro study

DOI: https://doi.org/10.25241/stomaeduj.2020.7(3).art.1


Introduction The aim of this study was to assess the cytotoxicity profiles of eight different fixed prosthetic restoration materials [Gold-based alloy (A), Chromium-Cobalt alloy (B) and Nickel-Chromium alloy (C), fiber-reinforced-resin-blocks (D), resin-nano-ceramic (E), lithium-disilicate-glass-ceramics (F), monolithic-zirconia (G) and feldspathic-ceramic (H)] by using cell culture methods on the L929 mouse fibroblast cells.
Methodology 36 disc-shaped samples of each test material were prepared (5x2mm, N=288). After sterilization, discs were placed in EMEM and incubated at 37°C. Mediums were collected and filtered from each of four samples in 1st and 7th days. After 24hours incubation, cells were treated with 100 µl medium extracts of materials. Viability of cells was measured after 48 hours. Cytotoxicity was assessed with XTT and xCELLigence tests. Apoptosis was analysed using Annexin-V/PI staining. All data were statistically analysed with One-way ANOVA and Tukey’s multiple range tests (p<0.05).
Results Considering the cell viability and apoptosis rate significant differences were found after the 1st and 7th days of incubation periods for each material group (p<0.05). Among the material groups significant differences were observed (p<0.05). F group showed the lowest cell viability and showed highest apoptosis rate (p<0.05). Along the entire test period, E group showed the highest cell viability and lowest apoptosis rate (p<0.05).
Conclusion All fixed restoration materials investigated in the study exposed various levels of cytotoxicity, with significant differences among the test groups.

Dental Alloys; CAD-CAM Materials; Cytotoxicity; Apoptosis; Fibroblasts.