Art-3-Marcelino

CARIOLOGY                                                                                                                                                    www.stomaeduj.com




THE USE OF ER:YAG LASER FOR DENTAL CARIES REMOVAL




                                                                                                                                                           Review Articles
                                   1a                                          1b                                          2c                        2d*
Geise dos Santos Marcelino              , José Humberto Ribeiro Lopes               , Juliana Jendiroba Faraoni                 , Pâmella Coelho Dias

1School of Dentistry, Faculdade Morgana Potrich (FAMP), Mineiros, Goiás, Brazil
2Restorative Dentistry Department, School of Dentistry, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil
a
  DDS, General Dentist; email: hisy74@gmail.com; ORCID-iD: https://orcid.org/0000-0003-0056-1580
b
  DDS, General Dentist; email: zehumberto96@gmail.com; ORCID-iD: https://orcid.org/0000-0001-5028-4425
c
 DDS, PhD, Researcher; email: jujfaraoni@yahoo.com.br; ORCID-iD: https://orcid.org/0000-0003-0945-4028
d
  DDS, MSc, PhD student; email: pamellacdias@hotmail.com; ORCID-iD: https://orcid.org/0000-0003-4886-3940
ABSTRACT                                                                               https://doi.org/10.25241/stomaeduj.2021.8(3).art.3

Background Carious tissue removal in enamel and dentin requires the use of sharp and resistant instruments.
New tools have appeared to optimize/facilitate dental treatment, among them the laser. Regarding laser
application for dental caries removal, the use of erbium laser doped with yttrium, aluminum, and garnet
(Er:YAG) stands out. The Er:YAG laser is excellent for hard tissues ablation since its wavelength of 2.940nm is
highly absorbed by water and hydroxyapatite.
Objective To review the application of the Er:YAG laser in dental caries removal, to present its advantages and
limitations in clinical practice, as well as to describe its action mechanism, and to compare its effectiveness
with different methods used to remove caries.
Data sources The search for articles to compose this literature review was carried out in the PubMed and
Embase databases.
Study selection Articles in English published between 2006 and 2021. The manual search included additional
articles and books; a total of 39 references were selected.
Data extraction Information from studies that evaluated the use of the Er:YAG laser to remove caries or
related this type of laser to other methods. Articles that evaluated characteristics of the dental structure, or
the influence of restorative materials after caries removal with the Er:YAG laser, were also considered.
Study selection Based on studies results, the Er:YAG laser presents itself as an alternative for caries removal
since it can remove demineralized tissue (selective ablation) without causing damage to the dental element.

KEYWORDS
Cariology; Dental Caries; Dental Caries Removal; Dentin; Lasers.

1. INTRODUCTION                                                                  Lasers can be classified, according to their
                                                                                 application, in low and high-power lasers [4]. Low-
Contemporary dentistry is based on early diagnosis,                              power lasers can aid analgesia, reduce inflammation
adequacy of the oral environment, and prevention                                 and stimulate tissue repair [3,5], while high-powered
of oral diseases. In recent years, new tools have                                ones are used in surgery, ablation of decayed tissue,
emerged to optimize/facilitate dental treatment,                                 and orthodontics [2,4,5]. There are also lasers used
including laser. Studies carried out over the years                              for photodynamic therapy and tissue fluorescence
have proven the effectiveness of laser therapy for                               diagnosis [4].
hard and soft tissues manipulation [1-3].                                        Regarding the application of laser for dental
The word LASER comes from the English acronym                                    caries removal, the use of erbium-doped yttrium,
Light Amplification by Stimulated Emission of                                    aluminum, and garnet laser (Er:YAG laser) stands
Radiation. Its mechanism of action is based on                                   out. This laser operates in a pulsed mode, and the
the emission of a collimated light beam of high                                  handpiece includes a spray of water to prevent
energy intensity and can be stimulated by solid,                                 tissue dryness and heat build-up, allowing energy to
liquid, or gaseous active medium. Lasers have                                    be absorbed efficiently [6].
different wavelengths, and this implies the variable                             Several types of research have evaluated the use of
phenomena that they can present: absorption,                                     lasers in dentistry, highlighting the erbium laser in
penetration, transmission, and diffusion. In dentistry,                          hard tissues [1,2,7,8]. This study aimed to perform a
the most desirable phenomenon is absorption, as it                               literature review about the application of the Er:YAG
will interact with living tissue, effectively exercising                         laser in dental caries removal and to present its
its different functions [4].                                                     advantages and limitations in clinical practice.
              OPEN ACCESS This is an Open Access article under the CC BY-NC 4.0 license.
              Peer-Reviewed Article
    Citation: Marcelino GDS, Lopes JHR, Faraoni JJ, Dias PC. The use of Er:YAG laser for dental caries removal. Stoma Edu J. 2021;8(3):173-183.
    Received: August 17, 2021; Revised: September 11, 2021; Accepted: September 27, 2021; Published: September 28, 2021
    *Corresponding author: Pâmella Coelho Dias, DDS, MSc, PhD student; Department of Restorative Dentistry, School of Dentistry, University of São
    Paulo, Avenida do Café, s/n, 14040-904, Ribeirão Preto, SP, Brazil; Tel./Fax: +55 16 3315-4016; e-mail: pamellacdias@hotmail.com
    Copyright: © 2021 the Editorial Council for the Stomatology Edu Journal.




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                    2. METHODOLOGY                                             have shown that chemical-mechanical methods
Review Articles                                                                like Carisolv® and Papacarie® are also effective in
                    The article search for this literature review utilized     removing caries [11,12].
                    the PubMed and Embase databases, and the                   The laser is another alternative that has been used in
                    selection included articles published between              the dental field as an effective method for removing
                    2006 and 2021. The terms used were “Er:YAG laser”          decayed tissue, being considered a conservative
                    AND “dental caries removal”. The inclusion criteria        method since such removal is selective. Besides,
                    included articles in English published between 2006        laser treatment promotes greater comfort during
                    and 2021 that evaluated the use of the Er:YAG laser        the surgical procedure and without causing pulpal
                    to remove caries or related this type of laser to other    damage [13-15]. The type of laser most used for this
                    methods. Articles that evaluated characteristics of        purpose is the yttrium-aluminum-garnet erbium
                    the dental structure, or the influence of restorative      laser (Er:YAG).
                    materials after removal of caries with Er:YAG laser,       The Er:YAG laser is excellent for the ablation of hard
                    were also considered. The search excluded: literature      tissues, as it has a wavelength of 2.940nm, which
                    reviews, monographs, case reports, studies with            is highly absorbed in water and hydroxyapatite.
                    bovine teeth, and studies with artificial or induced       The erbium laser creates microexplosions in the
                    demineralization. The manual search included ten           hydroxyapatite by vaporizing the water molecules
                    studies: three books, four original articles, and three    present in the hard tissues, which leads to the
                    reviews as they contained viable information to            breakdown of this tissue during the ablation
                    structure this literature review. Figure 1 shows the       process. This laser operates in a pulsed mode, and
                    flowchart detailing the selection of articles. In total,   the handpiece includes a spray of water to prevent
                    this review included 39 references.                        tissue dryness and heat accumulation, allowing
                                                                               the energy to be absorbed efficiently. Thus, its
                                                                               action occurs without tissue carbonization and
                                                                               with minimal generation of heat [6]. Its use was
                                                                               approved for these purposes by the Food and Drug
                                                                               Administration (FDA) in May 1997 [14].
                                                                               Research that used scanning electron microscopy
                                                                               (SEM) and thermographic study to evaluate the
                                                                               pulp temperature during the use of Er:YAG laser in
                     Figure 1. Flowchart of the article selection process.     deciduous and permanent teeth showed that in the
                                                                               same pulse of energy, ablation in dentin was more
                    3. LITERATURE REVIEW                                       effective than in enamel and that ablation and caries
                                                                               removal values were significantly higher in primary
                    Dental caries is described in the literature as a          teeth when comparing to permanent teeth, but
                    multifactorial disease influenced by genetic,              without exceeding the temperature of 5.5°C [7].
                    environmental, and behavioral characteristics, being       Eberhard et al. (2008) analyzed extracted decayed
                    a complex disease resulting from the accumulation          permanent molars in which they were sectioned
                    of specific acidogenic bacterial colonies present in       and treated with fluorescence-feedback controlled
                    the dental biofilm, capable of adhering to the tooth.      (FFC) Er:YAG laser or diamond tips. The use of the
                    These, in turn, produce acids from their metabolism        FCC Er:YAG laser at a threshold of 7U (units) resulted
                    using fermentable carbohydrates responsible for            in less dentin loss when compared to the use of
                    decreasing the pH on the dental surface, promoting         diamond tips [16].
                    the reduction of hydroxyapatite crystals and the           A randomized clinical trial compared the efficacy
                    widening of the intercrystalline spaces, leading           of the fluorescence-controlled Er:YAG laser and
                    to an increase in porosity and, consequently, the          the low-speed bur in removing decayed tissue in
                    emergence of the disease [9].                              adults. The results showed that the use of the FCC
                    Clinically, the active initial caries lesion appears       laser at a threshold of 7 and 8U promotes the same
                    on enamel as a white spot with a rough and                 effectiveness of the bur, presenting insignificant
                    opaque appearance. With the progression of                 numbers of remaining bacteria [17].
                    demineralization, this lesion forms a cavity that          One research evaluated different techniques
                    can progress to reach the dentin, which then starts        for caries removal and found no selectivity for
                    to show generally a yellow color, moist aspect, and        demineralized tissue using the Er:YAG laser. In this
                    softened consistency [9].                                  study, the laser showed significant variability in
                    The removal of decayed enamel and dentin tissue            the results, where several samples remained with
                    requires the use of strong and sharp instruments           amounts of remaining caries and others had tooth
                    to allow the proper preparation of the cavity. There       structure removed in excess [18].
                    are several instruments used for cavity preparation,       A study evaluating the rate of ablation and selectivity
                    such as hand instruments (chisels, dentin scoop) and       of healthy and demineralized enamel and dentin
                    rotary instruments (carbide burs with different types      promoted by a 30W (watts) diode-pumped Er:YAG
                    of active tip, diamond tips) [10]. Previous studies        laser operating on a pulse of 20-30µs (microseconds)



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showed that this laser has considerable potential for     surface and some dentinal tubules obliterated by




                                                                                                                     Review Articles
selective removal of dental caries [19].                  denatured collagen and surface contaminants;
Analysis of the removal of demineralized dentin           surfaces prepared with low and high-speed burs
using the FFC Er:YAG laser showed that dentin             showed a thick smear layer and no micro retentions
ablation occurred effectively at fluorescence control     [22].
values between 6U and 7U when measured by                 An in vitro study evaluated marginal microleakage
microCT (computed microtomography). While at              of cavities restored with glass ionomer, comparing
a value greater than 8U, the removal of decayed           Er:YAG laser with Apacaries gel and atraumatic
dentin was unsatisfactory [20].                           restorative technique (ART), and found a higher level
Kornblit et al. (2008) evaluated the effectiveness        of infiltration with the use of the laser to remove
of the Er:YAG laser in removing caries in deciduous       caries compared to the other methods [23].
and permanent molars and observed that ablation           Studies show that different techniques for removing
in areas infected by caries promoted maximum              decayed tissue influence the bond strength of
maintenance of the remaining structure. According         adhesive systems [24-27]. The study of Yildiz et al.
to these authors, the laser provided decontamination      (2013) concluded that the chemical-mechanical
of the affected area and improved retention of the        method or use of burs at low rotation compared
composite resin to the surface prepared with laser,       to the Er:YAG laser show better results in terms of
promoting better marginal enamel sealing [2].             bond strength for both self-etch and total-etch
Yonemoto et al. (2006) evaluated the DIAGNOdent®          adhesives [27]. In another study, using a 2-step self-
as a guide for caries removal using the Er:YAG laser.     etch adhesive system, the dentin surface prepared
Values set at 11-20 were able to remove caries            by the Er:YAG laser showed lower micro-tensile bond
preserving the affected dentin in vitro [21].             strength (µTBS) values compared to healthy dentin
An in vivo study used 120 primary teeth from              [24]. In the study of Sattabanasuk et al. (2006), the
children aged 5 to 9 years, divided into four groups:     bond strength values of a total-etch adhesive system
air rotor, Carisolv®, Papacarie®, and Er:YAG laser. The   were similar for the Er:YAG laser and steel bur [25].
results were visually and tactile observed, besides       Sirin Karaarslan et al. (2012) evaluated the micro-
having the values of the DIAGNOdent® pen and the          tensile bond strength of 3 types of adhesive systems
FLACC scale (Face, Legs, Activity, Cry, Consolability)    - Clearfil® SE Bond (2-step self-etch), G-Bond® (single-
to measure pain during the procedure. Air rotor and       step self-etch), and Adper® Single Bond 2 (2-step
laser were the most effective and efficient methods,      total-etch) after caries removal using a spherical steel
and laser and chemical-mechanical methods were            bur at low-speed, Carisolv® gel or the Er:YAG laser.
considered more comfortable [13].                         Based on the results, the techniques used to remove
Another in vivo study evaluated the FFC Er:YAG laser      decayed tissue showed significant differences in
for caries removal in pediatric patients. S. mutans and   bond strength between the adhesive systems. There
or Lactobacilli were found in 33.33% of the lesions.      was no significant difference in the bond strength of
In a total of 79 lesions, 14 contained S. mutans and      total-etch adhesive systems comparing laser and bur
15 contained Lactobacilli. The average log of colony-     groups. Using the laser, Adper® Single Bond 2 was
forming units (CFU) per sample was 0 for S. mutans        superior to the other adhesive systems, indicating
and Lactobacilli. The average time to perform the         that total-etch adhesives are the best option in this
procedure was 2.3±1.2min. Regarding pain, 93.8%           type of caries removal method [26].
of children considered laser usage comfortable [15].      The microhardness and chemical composition of
One research biochemically analyzed decayed and           dentin vary according to the applied caries removal
healthy teeth treated by the fluorescence-controlled      method. The chemical-mechanical technique
Er:YAG laser, where a layer of dentin was removed         (Carisolv®) showed lower microhardness of the
from the bottom of the preparation to determine           remaining dentin and a considerable number of
the presence of hydroxylysylpyridinoline (HP) and         samples with residual caries (20%) when compared
lysylpyridinoline (LP) collagen cross-links using         to the carbide bur and the Er:YAG laser (both 5%).
high-performance liquid chromatography. 100%              There was no significant difference in calcium and
HP and LP were found in decayed dentin and 0.33%          phosphorus rates of the three evaluated groups [28].
HP and 0.68% LP in healthy dentin. After caries           A comparative clinical study between the bur and the
removal, 0.84% HP and 1.26% LP were found at the          Er:YAG laser to remove caries from primary molars
5U fluorescence-control threshold and 1.56% HP            showed that the laser was less efficient than the bur
and 2.48% LP at 10U. The Er:YAG laser proved to           to remove caries. Regarding effectiveness, the two
be a viable method for removing the irreversibly          treatments were similar to remove caries in the pulp
denatured collagen present in decayed dentin [1].         wall, and the bur was better in the surrounding walls.
Scanning electron microscope was used to                  The composite resin restorations for both groups
investigate the morphological changes in the              remained satisfactory after one year of treatment [8].
hard dental tissues after caries removal and cavity       A double-blind clinical study, performed in
preparation using different methods: Er:YAG laser,        children aged 7-10 years, evaluated composite
Carisolv® gel, high-speed diamond burs, and low-          resin restorations in primary teeth performed after
speed micromotor steel burs. The dental surface           selective removal of necrotic dentin using an Er:YAG
after using the laser remained highly retentive,          laser and a carbide bur. Adhesive restorations did not
without smear layer residues and with the presence        suffer laser interference, and the SEM analysis showed
of exposed dentinal tubules. The samples treated          that laser group restorations showed a 10% gap in its
with Carisolv® gel presented a rough retentive            extension, and the group treated with a bur showed


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                    a 20% gap in the cavosurface margin after 12 months        from 45 patients. No adverse reactions and no or little
Review Articles     of follow-up [29].                                         pain were reported in most treated teeth (89.5%).
                    A longitudinal clinical study with four years of           Tooth preparation was successfully performed
                    follow-up evaluated the clinical longevity of              exclusively by laser in 94.7% of the cases, and the
                    composite resin restorations after selective caries        operative time was on average 49 seconds [36].
                    removal in permanent molars using the Er:YAG laser
                    or bur preparation with chlorhexidine as dentin            4. DISCUSSION
                    biomodifier. The Er:YAG laser group biomodified with
                    chlorhexidine presented a statistically significant        The Er:YAG laser to remove caries has been widely
                    difference for marginal adaptation criteria compared       studied in recent years, showing that it is a safe and
                    to the other groups; for secondary caries criteria and     comfortable method for the patient, minimizing the
                    clinical and radiographic evaluation of pulp vitality,     use of local anesthesia and maintaining pulp vitality
                    there was no statistically significant difference          [2,7,13,17].
                    between the evaluated groups. The authors                  Studies comparing different methods of caries
                    concluded that the method of caries removal and            removal found that the Er:YAG laser, together with
                    dentin biomodification did not influence the survival      the chemical-mechanical method, provided greater
                    rate of composite resin restorations [30].                 comfort and satisfaction for both the operator
                    Prabhakar et al. (2018) evaluated morphological            and the patient as they were less traumatic [13].
                    changes and the presence of bacterial deposits             According to a systematic review, chemomechanical
                    in primary decayed molars submitted to carious             methods are the best option for a minimally invasive
                    tissue removal by Carie-Care (chemical-mechanical          treatment [33]. In an in vitro study, the hand excavator
                    method), Er:YAG laser, and tungsten carbide                was the most effective technique to remove caries in
                    spherical bur. The results showed that the laser           deciduous teeth [37].
                    group was the most effective of the three, with fewer      Comparing caries removal effectiveness (capacity)
                    bacterial deposits and no smear layer formation [31].      between laser and carbide bur at low rotation,
                    A meta-analysis evaluated the Er:YAG laser to              the results of the two methods were similar for
                    remove caries and for cavity preparation in children       removing caries from the pulpal wall; the bur was
                    compared to the traditional mechanical method. This        more effective on the surrounding walls of primary
                    study evaluated seven randomized clinical trials and       molars [8]. Both techniques showed similar results
                    found that the laser requires more operative time but      regarding the presence of residual caries [28].
                    is less painful. There were no statistically significant   The adjusted value in lasers with fluorescence-
                    differences between the two types of treatment             feedback control influenced selectivity for carious
                    concerning retention, marginal adaptation, and             tissue in the studies evaluated in this literature
                    marginal discoloration of restorations [32].               review. Schwass et al. (2013) showed that the Er:YAG
                    A recent systematic review showed that the use of          laser with fluorescence control selected between 7U
                    burs, chemomechanical method, and the Er:YAG               and 8U was effective for removing demineralized
                    laser are efficient for caries removal, reduction of       dentin [20], as well as studies by Dommisch et al.
                    bacteria in the tooth cavity, and do not compromise        (2008) [17] and Eberhard et al. (2008) [16], where
                    the clinical performance of restorations [33].             the Er:YAG laser with fluorescence control at levels
                    An in vitro study compared the FFC Er:YAG laser            of 7U and 8U promoted caries removal similar to
                    with the Er:YAG laser in three different pulses (super     that obtained with the conventional bur, generating
                    short, medium short, and short pulse) regarding            greater comfort and wellness to the patient. The
                    the efficiency of removing cariogenic bacteria and         use of a laser with fluorescence control set to 9U
                    carious dentin and dentin temperature during               and 10U did not remove all decayed tissue [17].
                    ablation. The results showed that no experimental          Contrasting these results, Neves et al. (2011) did not
                    group had bacterial contamination after treatment.         find selectivity when analyzing samples prepared
                    In the groups with varied pulses of laser energy, the      with the FFC Er:YAG laser at the threshold of 7U,
                    dentin temperature was significantly higher than the       where some specimens were overprepared, and
                    FFC laser [34].                                            others continued with decayed tissue. In this study,
                    A study that evaluated and compared the Er:YAG             the laser was the evaluated method that presented
                    laser, the tungsten bur, and the polymer bur in            the least minimally invasive potential [18].
                    caries removal showed no difference between the            Regarding the morphological changes generated on
                    treatment time comparing the three methods.                the treated dental surface, the laser did not promote
                    Histological analysis showed that all groups               thermal damage and also left the surface highly
                    effectively removed the infected dentin and the laser      retentive and without the presence of a smear layer;
                    group showed a regular 5µm thick layer of denatured        while the use of burs in both high and low rotation
                    collagen. The group treated by the tungsten bur            promoted surfaces with a thick smear layer and
                    presented a smear layer, and the polymer bur group         absence of microretentions; the use of polymer burs
                    showed an affected dentin surface layer [35].              left an affected dentin layer [22,35].
                    Matsumoto et al. (2007) clinically evaluated the           Despite the advantages of using laser as a method
                    applicability of the Er:YAG laser on 95 decayed teeth      of removing decayed tissue, clinical studies have



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Er:YAG laser for caries removal
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shown variation in clinical time, with laser consuming           A 12-month follow-up of a randomized clinical




                                                                                                                                      Review Articles
about 3x more time than the use of burs [17] with                trial with a split-mouth design showed that teeth
an average treatment duration of 2.3±1.2min [15].                with decayed tissue removed by both laser and
Other studies have also shown a longer average                   bur and restored with a 2-step total-etch adhesive
treatment time with the use of laser, but with a                 system and composite resin maintained satisfactory
minor difference: 110s [8] or 49s [36] for the Er:YAG            restorative treatment [8]. In a double-blind clinical
laser and 55s [8] for the low-speed carbide bur.                 study, the restorations of the laser group showed a
Another disadvantage found in one of the selected
                                                                 lower percentage of a gap than the group treated
studies was the presence of marginal infiltration in
                                                                 by bur in the analysis performed by SEM at the same
decayed teeth ablated by laser and restored with
glass ionomer [23].                                              follow-up time [29].
The bond strength of adhesive systems influenced                 In a biochemical analysis of dentin collagen on
the method used to remove caries [26]. According                 decayed surfaces treated with erbium laser through
to the results, the authors suggest choosing a                   SEM, the percentage of dehydrated collagen and
conventional adhesive system after caries removal                decayed dentin reduced after laser treatment,
by the Er:YAG laser and a self-etch adhesive system              becoming similar to healthy dentin [1]. In the study
after using a chemical-mechanical method. These                  of Krause et al. (2008), 42.9% of the samples had
results corroborate the study by Neves et al. (2011)             bacterial residues, but in only 7.1%, the bacterial
that found lower micro-tensile bond strength using a             count was greater than 100 CFU (colony forming
2-step self-etch adhesive after caries removal by the            units) [15].
Er:YAG laser compared to the chemical-mechanical                 The pulp response to the application of external
method and the use of conventional burs [24]. In                 heat was evaluated in an in vivo study using rhesus
another study, the use of a chemical-mechanical                  monkeys [39]. The results showed that a temperature
technique or usage of burs at low-speed compared
                                                                 increase of 2.2°C does not cause pulp changes and
to Er:YAG laser showed better results in terms of
                                                                 that increase of 5.5°C allowed pulp repair for most
bond strength for both self-etch and total-etch
adhesive systems [27]. Other studies showed that the             specimens (75%). In several studies, the use of the
Er:YAG does not influence the bond strength value                Er:YAG laser has proven not to generate thermal
of a total-etch adhesive system [25, 38]; however, the           damage to the dental structure, not exceeding the
study of Sattabanasuk et al. (2006) showed higher                temperature increase above the threshold tolerated
bond strength values for Er:YAG laser compared                   by the dental pulp [2,7,19,34].
to steel bur evaluating a self-etch adhesive system              Table 1 summarizes the 29 studies selected by
(Clearfil Protect Bond) [25].                                    Pubmed and Embase.
 Author (Year)       Substrate         Type of          Evaluated               Er:YAG laser                  Conclusion
                                        study          parameters              specifications
Al-Batayneh et   Sound and carious   In vitro      - Laser ablation in     - Wavelength: 2.94µm      - Laser creates greater crater
al. (2014) [7]   enamel and dentin   comparative   sound enamel and        - Pulse energy: 200mJ     depths in dentin than enamel
                 from 40 primary e   study         dentin                  - Pulse duration: 250µs   for both types of teeth
                 40 permanent                      - Comparison            - Frequency: 10Hz         - Laser is more efficient than
                 extracted teeth                   between the Er:YAG      - Power output: 6W        the rotary bur to remove
                                                   laser and the rotary                              caries in primary teeth
                                                   bur for carious                                   - There was no significant
                                                   removal                                           difference between
                                                   - Surfaces changes                                both methods regarding
                                                   through SEM                                       permanent teeth
                                                                                                     - Dental ablation did not
                                                                                                     exceed 5.5°C
                                                                                                     - SEM: dentin ablation with
                                                                                                     no smear layer
Baraba et al.    60 teeth with       In vitro      Caries removal          FCC laser:                -Ablated specimens were
(2018) [34]      dentin caries and   comparative   efficiency of the FFC   -Pulse energy: 350mJ      bacteria-free
                 12 teeth without    study         Er:YAG laser and        (enamel) and 250mJ        -All laser evaluated were
                 caries lesion.                    different pulses of     (dentin)                  efficient for caries removal
                                                   the Er:YAG laser        -Pulse duration: 400µs    -FCC laser group presented
                                                   -PCR analysis           -Frequency: 4Hz           the lowest average
                                                   -Thermal alterations    -Threshold: 7U            temperature
                                                                           Laser with different
                                                                           pulses:
                                                                           -Pulses: 50µs, 100µs,
                                                                           and 300µs
                                                                           -Pulse energy: 350mJ
                                                                           (enamel) and 250mJ
                                                                           (dentin)
                                                                           -Frequency: 10Hz
                                                                           -Non-contact mode
                                                                           -Distance: 7mm




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Review Articles     Bohari et al.
                    (2012) [13]
                                       120 carious
                                       primary teeth
                                                         In vivo
                                                         comparative
                                                                          -Efficiency, efficacy,
                                                                          and level of pain
                                                                                                   - Wavelength: 2.94µm
                                                                                                   -Pulse energy: 200mJ
                                                                                                                                -Air rotor and laser were faster
                                                                                                                                and more efficient
                                       from children     study            of 4 methods of          -Frequency: 20Hz             -Carisolv®, Papacarie®, and
                                       aged 5 to 9                        caries removal: air      -Power output: 4W            laser were less painful
                                                                          rotor, Carisolv®,        -Contact mode
                                                                          Papacarie®, and
                                                                          Er:YAG laser
                    Cardoso et al.     Carious primary   Systematic       -Dental caries                                        -All methods are efficient in
                    (2020) [33]        teeth             review           removal efficacy,                                     reducing CFU count and in
                                                                          treatment                                             removing caries
                                                                          time, need of                                         -Chemomechanical methods
                                                                          anesthesia, CFU                                       showed to be the best
                                                                          count, restoration                                    option for minimally invasive
                                                                          performance,                                          treatments
                                                                          and pain                                              -Conventional methods
                                                                          perception among                                      promote faster treatment, and
                                                                          conventional,                                         Er:YAG is faster than Carisolv®
                                                                          chemomechanical,                                      and Papacarie®
                                                                          and laser methods.                                    -Chemomechanical and laser
                                                                                                                                require less anesthesia and are
                                                                                                                                also less painful
                                                                                                                                -Restorations were not
                                                                                                                                affected by any of the caries
                                                                                                                                removal methods
                    Cebe et al.        10 permanent      In vitro         - Microtensile           - Wavelength: 2.94µm         -The Er:YAG laser did not
                    (2017) [38]        molars with       comparative      strength of a total-     -Power output: 3.5W          impair the bond strength of a
                                       proximal caries   study            etch adhesive            -Pulse duration: 300µs       total-etch adhesive system
                                                                          system after caries      (short pulse mode)
                                                                          removal by the           -Frequency: 10Hz
                                                                          Er:YAG laser and bur     -1mm distance
                                                                                                   -Energy density: 44J/cm2
                    Celiberti et al.   80 deciduous      In vitro         - Time and               - Wavelength: 2.94µm         - Carbide bur was the fastest
                    (2006) [37]        molars with       comparative      caries removal           - Pulse energy: 200mJ        and the Er:YAG laser the
                                       dentin caries     study            effectiveness            - Frequency: 4Hz             slowest technique
                                                                          and selectivity          - Non-contact mode           - Polymer bur and laser
                                                                          of carbide bur,          - Working distance:          left large amounts of
                                                                          Er:YAG laser, hand       12mm                         underprepared areas
                                                                          excavator, and                                        - Carbide bur was the least
                                                                          polymer bur                                           conservative method
                                                                                                                                - Hand excavator was the most
                                                                                                                                effective technique to remove
                                                                                                                                caries in deciduous teeth
                    Dommisch et        102 teeth with    Randomized       - Efficiency, CFU        - Wavelength: 2.94µm         - FCC laser at the threshold
                    al. (2008) [17]    active caries     clinical trial   count, level of pain     - Pulse energy: 250mJ        of 7 and 8U and carbide
                                       from 26 adult                      between the FCC          - Frequency: 4Hz             burs showed similar results
                                       patients                           Er:YAG laser and         - Non-contact mode           regarding S. mutans and
                                                                          carbide burss            - Working distance: 10-      Lactobacilli CFU
                                                                                                   20mm                         - FCC laser was more
                                                                                                   - Threshold: 7, 8, 9, and    comfortable but significantly
                                                                                                   10U                          more time consuming
                                                                                                                                compared to carbide burs
                    Eberhard et        165 permanent     In vitro         - Cavity extension       - Wavelength: 2.94µm         - the FCC Er:YAG laser was
                    al. (2008) [16]    molars with       comparative      after caries removal     - Pulse energy: 250mJ        more conservative than the
                                       dentin caries     study            from 2 methods:          - Pulse repetition rate: 4   bur at a threshold of 7U
                                                                          bur and FCC laser        pulses/s                     - There was no difference
                                                                          in contact and non-      - contact and non-           between the laser in contact
                                                                          contact mode             contact mode                 and non-contact mode
                                                                                                   - Working distance: 12-
                                                                                                   15mm
                                                                                                   - Threshold: 6, 7, and 8U
                    Jepsen et al.      210 carious and   In vitro         - Percentage of          - Wavelength: 2.94µm         - the FCC Er:YAG laser showed
                    (2008) [1]         60 caries-free    study            denatured collagen       - Pulse energy: 250mJ        less denatured collagen at a
                                       teeth                              in dentin                - Pulse repetition rate: 4   threshold of 5U
                                                                          - Morphological          pulses/s                     - SEM analysis showed no
                                                                          aspects of dentin        - non-contact mode           smear layer and tubule
                                                                          after laser ablation     - Working distance:          exposure at the laser group
                                                                                                   adjusted by the pilot        and presence of smear layer
                                                                                                   laser beam                   and smear plug at the bur
                                                                                                   - Threshold: 5 and 10U       group
                                                                                                                                - Transmission electron
                                                                                                                                microscope (TEM) analysis
                                                                                                                                showed no bacteria in the
                                                                                                                                dentin surface after laser
                                                                                                                                ablation




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                                                                                                                                               Review Articles
Juntavee         Primary second       In vitro         - Influence of          - Pulse energy: 260mJ      - the Er:YAG laser promoted
et al. (2013)    molars with          comparative      Apacaries gel,          - Frequency: 30Hz          significant higher microleakage
[23]             occlusal caries      study            Er:YAG laser, and       - Pulse mode               than Apacaries and spoon
                                                       spoon excavator                                    excavator in ionomer
                                                       as caries removal                                  restorations
                                                       methods in
                                                       the marginal
                                                       microleakage of
                                                       glass ionomer
                                                       restorations
Katirci et al.   Permanent molars     In vitro         - Effectiveness         - Pulse energy: 250mJ      - Carbide bur and Er:YAG
(2016) [28]      with occlusal        comparative      of three caries         - Frequency: 4Hz           laser had a similar outcome
                 caries               study            removal methods by      - Non-contact mode         regarding caries removal
                                                       stereomicroscopic       - Working distance:        - Carisolv® was less effective
                                                       observations and        10mm                       than the carbide bur and the
                                                       microindentation                                   Er:YAG laser and presented
                                                       hardness                                           remaining dentin with a lower
                                                       measurement                                        hardness compared to the
                                                       - Chemical                                         other methods
                                                       composition of the                                 - There were no differences in
                                                       residual dentin                                    the calcium and phosphate
                                                                                                          ratio among the three methods
Kornblit et      30 carious teeth   Clinical trial     - Possible              - Wavelength: 2.94µm       - Children treated with Er:YAG
al. (2008)       from children aged                    postoperative           - Pulse duration: 140µs    did not show any pain or
[2]              4 to 12s                              complications after     - Pulse energy: 120 to     sensitivity 7 and 28 days after
                                                       caries removal with     200mJ                      the treatment
                                                       the Er:YAG laser        - Frequency: 2 to 20Hz
                                                                               - focus mode
                                                                               - Working distance: 0.8
                                                                               to 1cm
Krause et        79 carious lesions Clinical trial     - Efficacy of the FCC   - Wavelength: 2.94µm       - Treatment duration was
al. (2008)       from children aged                    Er:YAG laser            - Pulse duration: 400µs    2.3±1.2 min
[15]             3 to 12                               - CFU count after       - Pulse energy: 250mJ      - 93.8% of the children
                                                       laser treatment         - Frequency: 4Hz           considered the laser treatment
                                                       - Children              - non-contact mode         comfortable
                                                       perceptions during      - Working distance:        - After laser ablation, 42.9% of
                                                       treatment               12cm                       the samples showed residual
                                                                               - Threshold: 7U            bacteria; however, only 7.1%
                                                                                                          presented more than 100 CFU/
                                                                                                          sample
Li et al.        Carious teeth from   Meta-            - Duration of                                      - Caries removal with laser is
(2019) [32]      children             analysis         treatment                                          more time consuming than the
                                                       - Pain perception                                  use of bur
                                                       - Success of                                       - the Er:YAG laser is less painful
                                                       restorations                                       than -the bur
                                                                                                          - There were no statistical
                                                                                                          differences in complete
                                                                                                          restoration retention, marginal
                                                                                                          discoloration, and marginal
                                                                                                          adaptation between Er:YAG
                                                                                                          laser and bur
Matsumoto        Carious teeth from   Clinical trial   - Pain, discomfort,     - Wavelength: 2.94µm       - Laser showed: low rate of
et al. (2007)    adults                                assessment during       - Pulse energy: 700mJ      pain during treatment, no
[36]                                                   cavity preparation,     - Frequency: 8Hz           discomfort, ample efficacy,
                                                       prognosis factor,                                  substantial efficiency, good
                                                       and overall clinical                               prognosis after three months
                                                       evaluation                                         of follow-up, and mean of
                                                                                                          treatment duration of 49s
Medioni et       Carious molars       In vitro         - Effectiveness of      - Wavelength: 2.94µm       - Procedure time was similar for
al. (2016)       and premolars        comparative      - the Er:YAG laser,     - Pulse energy: 375mJ      all the three methods
[35]                                  study            carbide bur, and        - Pulse duration: 50µs     - Histological analysis showed
                                                       polymer bur for         - Frequency: 10Hz          smear layer in the specimens
                                                       caries removal          - Quasi-contact mode       treated with carbide bur,
                                                                                                          denatured collagen in the laser
                                                                                                          group, and a layer of affected
                                                                                                          dentin in the polymer bur
                                                                                                          group
                                                                                                          - All methods removed the
                                                                                                          infected dentin
Neves et al.     Carious molarss      In vitro         - Influence of the 7    - Pulse energy: 250mJ      - Remaining denting from the
(2011) [24]                           comparative      methods for caries      - Pulse repetition rate:   FCC Er:YAG laser group showed
                                      study            removal in the          4 pulses/s                 lower µTBS values
                                                       bonding capacity        - Non-contact mode         - Carisolv® showed the best
                                                       of the remaining        - Threshold: 7U            results regarding µTBS,
                                                       dentin                                             followed by carbide bur + caries
                                                                                                          detector



Stoma Edu J. 2021;8(3):173-183                                                             pISSN 2360-2406; eISSN 2502-0285                     179
                    Marcelino GDS, et al.
www.stomaeduj.com

Review Articles     Neves et al.
                    (2011) [18]
                                      Carious molars    In vitro
                                                        comparative
                                                                         - Caries removal
                                                                         effectiveness and
                                                                                                 - Pulse energy: 250mJ
                                                                                                 - Pulse repetition rate:
                                                                                                                               - Chemomechanical methods
                                                                                                                               + metal excavators showed the
                                                        study            minimally invasive      4 pulses/s                    best results
                                                                         potential of 9          - Non-contact mode            - the FCC Er:YAG laser showed
                                                                         methods for caries      - Threshold: 7U               the most variable results:
                                                                         removal by microCT                                    specimens with over and others
                                                                                                                               with under preparation
                                                                                                                               - the FCC Er:YAG laser did not
                                                                                                                               prove to be a selective method
                                                                                                                               for caries removal
                    Polizeli et al.   48 primary        Double-          - Salivary cortisol     - Medium short pulse          - Salivary cortisol levels were
                    (2019) [29]       molars with       blind,           levels and clinical     mode                          similar for laser and bur
                                      occlusal and      randomized       performance of          - Pulse energy: 250mJ         - There was no difference
                                      proximal caries   clinical trial   restorations after      - Frequency: 4Hz              regarding marginal adaptation,
                                      from children                      caries removal by       - non-contact mode            retention, discoloration,
                                      aged 7 to 10                       the Er:YAG laser        - Working distance:           and secondary caries for
                                                                         compared to             7cm                           restorations from both groups
                                                                         carbide bur                                           after one year of follow-up
                    Prabhakar et      Carious primary   In vitro         - Morphological         - Wavelength: 2.94µm          - the Er:YAG laser showed a
                    al. (2018) [31]   molars            comparative      changes                 - Pulse energy: 200mJ         minor quantity of bacterial
                                                        study            -Bacterial deposits     - Energy density: 22.5J/      deposits compared to
                                                                                                 cm2 for 10 pulse/s            Carie-Carie and carbide bur;
                                                                                                 - Non-contact mode            Carie-Carie presented greatly
                                                                                                                               bacterial deposits
                                                                                                                               - Morphological changes:
                                                                                                                               Carbide bur – thin smear layer
                                                                                                                               and few open tubules; Er:YAG
                                                                                                                               laser: irregular rugged surface,
                                                                                                                               no smear layer, opened tubules;
                                                                                                                               Carie-Carie – rough surface,
                                                                                                                               smear layer, obliterated tubules
                    Sattabanasuk      Carious third     In vitro         - Influence of 3        - Pulse energy: 180mJ         - The total-etch adhesive
                    et al. (2006)     molars            comparative      methods for caries      - Frequency: 2Hz              system (OptiBond Solo Plus)
                    [25]                                study            removal and two         - Non-contact mode            showed similar results in all
                                                                         types of adhesive                                     groups (steel bur, laser, and SiC
                                                                         systems in the                                        paper)
                                                                         bonding capacity                                      - The self-etch adhesive system
                                                                         of the remaining                                      (Clearfil Protect Bond) showed
                                                                         dentin                                                lower bond strength in dentin
                                                                                                                               treated with steel bur
                                                                                                                               -Only the laser group showed
                                                                                                                               similar bond strength for the
                                                                                                                               two tested adhesive systems
                    Schwass et al.    Teeth with        In vitro         - Different             - Pulse energy: 600mJ         - Feedback control values
                    (2013) [20]       proximal dentin   comparative      thresholds of           (enamel) and 250mJ            higher than 8U did not remove
                                      caries            study            FCC Er:YAG in the       (dentin)                      infected caries efficiently
                                                                         selectivity of caries   - Frequency: 10Hz             - Threshold for conservative
                                                                         removal                 (enamel) and 4Hz              caries removal lies between 7
                                                                                                 (dentin)                      and 8U
                                                                                                 - Pulse duration: 400µs
                                                                                                 - Threshold: 4, 5, 6, 7, 8,
                                                                                                 9, 10, 12, 16, and 20U
                                                                                                 - Non-contact mode
                    Sirin             Carious molars    In vitro         - Influence of 3        - Wavelength: 2.94µm          - The total-etch adhesive
                    Karaarslan et                       comparative      methods for caries      - Power output: 3.5W          system: similar bond strength
                    al. (2012) [26]                     study            removal and three       - Pulse duration: 300µs       values for steel bur and laser
                                                                         adhesive systems        (short pulse mode)            and lower for Carisolv®
                                                                         in the bonding          - Frequency: 10Hz             - One-step and two-step self-
                                                                         capacity of the         - 1mm distance                etch adhesive systems: similar
                                                                         remaining dentin        - Energy density: 44J/        bond strength values for all
                                                                                                 cm2                           three methods
                                                                                                                               - the bur group: all adhesive
                                                                                                                               systems had the same behavior
                                                                                                                               - the laser group: total-etch
                                                                                                                               adhesive showed the higher
                                                                                                                               µTBS values
                                                                                                                               - Carisolv®: two-step self-etch
                                                                                                                               adhesive showed the best
                                                                                                                               results




 180                Stoma Edu J. 2021;8(3):173-183                                                             pISSN 2360-2406; eISSN 2502-0285
Er:YAG laser for caries removal
                                                                                                                                               www.stomaeduj.com




                                                                                                                                             Review Articles
Tsanova         Carious teeth      In vitro         - Ultrastructural         - Pulse energy: 400mJ       - Er:YAG laser: irregular and
& Tomov         from adults        comparative      changes in substrates     - Frequency: 20Hz           rough dentin surface with no
(2010) [22]                        study            treated with Er:YAG       - Power output: 8W          smear layer, exposed tubules;
                                                    laser, Carisolv®,                                     retentive enamel
                                                    diamond bur, steel                                    - Carisolv®: dentin with rough
                                                    bur                                                   and granular aspect, retentive
                                                                                                          surface
                                                                                                          The diamond bur: thin and
                                                                                                          smooth smear layer in most
                                                                                                          regions and absence in a few
                                                                                                          others, opened tubules
                                                                                                          The steel bur: smear layer and
                                                                                                          obliterated tubules
Valério et      Carious            Split-mouth      - Er:YAG laser            - Pulse energy: 250mJ       - The carbide bur is faster and
al. (2016)      primary            randomized       effectiveness             - Frequency: 4Hz            more effective to remove caries
[8]             molars from        clinical trial   compared to carbide       - Non-contact mode          in the surrounding walls than
                children aged                       bur                       - 7mm distance              Er:YAG laser
                6 to 10                             - CFU count               - Energy density: 39J/cm2   - CFU for S. mutans and
                                                    - Restoration                                         Lactobacilli sp. was similar for
                                                    longevity                                             both methods
                                                                                                          - One-year follow-up showed
                                                                                                          no differences in restoration
                                                                                                          longevity for both groups
Valério et      Carious            Longitudinal     - Influence of Er:YAG     - Medium short pulse        - The clinical longevity of the
al. (2020)      molars from        clinical trial   and carbide bur           mode                        restorations was affected
[30]            children aged                       associated with           - Pulse energy: 260mJ       neither by the method for
                8 to 12                             chlorhexidine at the      - Frequency: 4Hz            caries removal nor by the
                                                    longevity of adhesive     - Non-contact mode          dentin biomodification with
                                                    restorations after four   -12mm distance              chlorhexidine at four years of
                                                    years of follow-up        - Energy density: 41J/cm2   follow-up
Yan et al.      Teeth with         In vitro study   - Potential of a diode    - Pulse duration: 20-30µs   - Ablation with minor thermal
(2015) [19]     occlusal caries                     pumped solid-state        - Frequency: 500Hz          damage
                                                    (DPSS) Er:YAG laser for   - Power output: 30W         - Higher ablation rate in
                                                    caries removal                                        demineralized surfaces
                                                                                                          compared to the sound ones
Yildiz et al.   Carious            In vitro         - Influence of 3          - Wavelength: 2.94µm        - Laser presented lower µTBS
(2013) [27]     primary molar      comparative      methods for caries        - Power output: 3.5W        values than carbide bur and
                teeth              study            removal and two           - Pulse duration: 300µs     Carisolv® groups regardless of
                                                    adhesive systems in       (short pulse mode)          the adhesive system used
                                                    the bonding capacity      - Frequency: 10Hz           (one-step self-etch and two-
                                                    of the remaining          - 1mm distance              step total-etch)
                                                    dentin                    - Energy density: 44J/cm2   - Bur and Carisolv® presented
                                                                                                          the best results for both types
                                                                                                          of adhesive systems
Yonemoto        Molars with        In vitro study   - Values of               - Pulse energy: 150-200mJ   - Laser + DIAGNOdent® values
et al. (2006)   occlusal                            DIAGNOdent® that          (enamel) and 50-150mJ       ≤10: overpreparation; laser +
[21]            dentin caries                       could be used as a        (dentin)                    DIAGNOdent® values of 11-
                                                    guide for the removal     - Pulse duration: 200µs     20: removal of the outer layer
                                                    of the outer layer of     Frequency: 10, 20, 25 or    dentin; laser + DIAGNOdent®
                                                    carious dentin with       30Hz                        values of 21-30: partial
                                                    Er:YAG laser                                          removal of the outer layer
                                                                                                          dentin; however, there are
                                                                                                          some limitations for clinical
                                                                                                          application

 Table 1. Summary of the selected articles.
5. CONCLUSION                                                             Despite the advantages, its use requires more clinical
                                                                          time compared to the use of burs. Furthermore, the
The Er:YAG laser is a viable alternative for the treat-                   cost of the equipment, despite not having been
ment of caries since it can remove demineralized                          addressed in the review, can also be considered
                                                                          a limiting factor. In general, the Er:YAG laser is
tissue without causing damage to the dental
                                                                          as effective as the conventional and chemical-
element, in addition to providing greater comfort for                     mechanical methods for selective caries removal.
the patient due to the absence of noise, vibrations,
and pressures during removal of decayed tissue and                        ACKNOWLEDGMENTS
less need for anesthetic administration in most cases.                    None.

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 182                Stoma Edu J. 2021;8(3):173-183                                                                 pISSN 2360-2406; eISSN 2502-0285
Er:YAG laser for caries removal
                                                                                                                                                   www.stomaeduj.com



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                                                                       Geise dos Santos MARCELINO
                                                                                          DDS, General Dentist
                                                                            Department of Restorative Dentistry
                                                                                           School of Dentistry
                                                                             Faculdade Morgana Potrich (FAMP)
                                                                                          Mineiros, Goiás, Brazi

CV
Geise dos Santos Marcelino graduated from FAMP, Mineiros, Goiás, Brazil, and is a student of the Specialization course in
Orthodontics at the Graduate Center in Dentistry – CIOG, Goiânia, Goiás. Her academic interests include Hospital Dentistry and
Pediatric Dentistry. She works as a general dentist in a private practice in Senador Canedo, Goiás and at the PSF in Taquaral de
Goiás, Goiás, Brazil.




Questions
1. Which caries removal method is the least conservative?
qa. Mechanical removal with dentin scoop;
qb. Diamond tips and air rotor;
qc. Er:YAG laser;
qd. Use of a chemical-mechanical method.

2. Which is the Er:YAG laser wavelength?
qa. 2.940nm;
qb. 2.840nm;
qc. 2.490nm;
qd. 2.480nm.

3. Which sentence is correct?
qa. Er:YAG is a selective method for caries removal;
qb. Chemical-mechanical methods cause more pain for the patients;
qc. Er:YAG can affect pulp vitality;
qd. Carbide burs cannot be used in children.

4. In dentistry, which one is the most desirable phenomenon of the laser?
qa. Diffusion;
qb. Transmission;
qc. Absorption;
qd. Penetration.




Stoma Edu J. 2021;8(3): 173-183                                                            pISSN 2360-2406; eISSN 2502-0285                       183